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Single-molecule techniques : a laboratory manual / edited by Paul R. Selvin, Taekjip Ha

Ouvrage
Auteur secondaire: Selvin, Paul R., EditeurHa, Taekjip, EditeurLangue : anglaisPays : Etats-Unis.Publication : Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, cop. 2008Description: 1 vol. (vii-507 p.), ill., 29 cmISBN : 9780879697761; 0-87969-776-8; 978-0-87969-775-4; 0-87969-775-X.Note de contenu : The new era of biology in singulo / T. Ha and P.R. Selvin Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin, T. Lougheed, M.T. Hoffman, H. Park, H. Balci, B.H. Blehm, and E. Toprak Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich Alternating-laser excitation of single molecules / A.N. Kapanidis, M. Heilemann, E. Margeat, X. Kong, E. Nir, and S. Weiss Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang, Y. Sun, M.E. Quinlan, J.N. Forkey, and Y.E. Goldman Imaging gene expression in living cells at the single-molecule level / J. Xiao, J. Elf, G.-W. Li, J. Yu, and X.S. Xie Single-virus tracking in live cells / M.J. Rust, M. Lakadamyali, B. Brandenburg, and X. Zhuang Ultrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan Imaging real-time gene expression in living systems / A.L. Wells, J.S. Condeelis, R.H. Singer, and D. Zenklusen Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille Optical traps to study properties of molecular motors / J.A. Spudich, S.E. Rice, R.S. Rock, T.J. Purcell, and H.M. Warrick High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa, T. Komori, T. Ariga, T. Okada, M. Morimatsu, Y. Ishii, and T. Yanagida Single-molecule studies using magnetic traps / T. Lionnet, J.-F. Allemand, A. Revyakin, T.R. Strick, O.A. Saleh, D. Bensimon, and V. Croquette Probing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J. A. Spudich Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder, P.C. Blainey, S. Kim, and X.S. Xie Bibliographie : Notes bibliogr. Index.Sujet - Nom commun: Biologie moléculaire | Microscopie de fluorescence | Microscopie à force atomique | Structure moléculaire | Biomolecules -- Analysis -- Laboratory manuals | Ultrastructure (Biology) -- Laboratory manuals | Molecular biology -- Laboratory manuals | Biomolecules -- Analysis -- Laboratory manuals | Fluorescence microscopy -- Laboratory manuals | Atomic force microscopy -- Laboratory manuals | Ultrastructure (Biology) -- Laboratory manuals
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Notes bibliogr. Index

The new era of biology in singulo / T. Ha and P.R. Selvin Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin, T. Lougheed, M.T. Hoffman, H. Park, H. Balci, B.H. Blehm, and E. Toprak Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich Alternating-laser excitation of single molecules / A.N. Kapanidis, M. Heilemann, E. Margeat, X. Kong, E. Nir, and S. Weiss Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang, Y. Sun, M.E. Quinlan, J.N. Forkey, and Y.E. Goldman Imaging gene expression in living cells at the single-molecule level / J. Xiao, J. Elf, G.-W. Li, J. Yu, and X.S. Xie Single-virus tracking in live cells / M.J. Rust, M. Lakadamyali, B. Brandenburg, and X. Zhuang Ultrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan Imaging real-time gene expression in living systems / A.L. Wells, J.S. Condeelis, R.H. Singer, and D. Zenklusen Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille Optical traps to study properties of molecular motors / J.A. Spudich, S.E. Rice, R.S. Rock, T.J. Purcell, and H.M. Warrick High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa, T. Komori, T. Ariga, T. Okada, M. Morimatsu, Y. Ishii, and T. Yanagida Single-molecule studies using magnetic traps / T. Lionnet, J.-F. Allemand, A. Revyakin, T.R. Strick, O.A. Saleh, D. Bensimon, and V. Croquette Probing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J. A. Spudich Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder, P.C. Blainey, S. Kim, and X.S. Xie