Single-Molecule Fluorescent Particle Tracking Single-Molecule Analysis of Biomembranes Single-Molecule Imaging in Live Cells Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy Single-Molecule FRET: Methods and Biological Applications Single-Molecule Enzymology Single-Molecule Studies of Rotary Molecular Motors Fluorescence Correlation Spectroscopy in Living Cells Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores Nanopores: Generation, Engineering, and Single-Molecule Applications Single-Molecule Manipulation Using Optical Traps Magnetic Tweezers for Single-Molecule Experiments Folding of Proteins under Mechanical Force Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy Probing Single Membrane Proteins by Atomic Force Microscopy High-Speed Atomic Force Microscopy Recognition Imaging Using Atomic Force Microscopy Atomic Force Microscopy of Protein–Protein Interactions A New Approach to Analysis of Single-Molecule Force Measurements Single-Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation

The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes: Experimental techniques to monitor and manipulate individual biomolecules The use of single-molecule techniques in super-resolution and functional imaging Single-molecule studies of physical properties of biomolecules Single-molecule enzymology and biochemistry Single molecules in the membrane Single-molecule techniques in living cells Integration of single-molecule biophysics and nanoscience

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