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010			_a9781493986934 _brel.
010			_a978-1-4939-9368-0 _bbr.
010			_z978-1-4939-8695-8 _be-book
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100			_a20181207d2018 k y0frey50 ba
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181			_6z01 _ctxt _2rdacontent
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182			_6z01 _cn _2rdamedia
182		1	_6z01 _an
183		1	_6z01 _anga _2RDAfrCarrier
200	1		_aMicrobial proteomics _emethods and protocols _fedited by Dörte Becher,...
214		0	_aNew York _cHumana Press
214		4	_dC 2018
215			_a1 vol. (XV-344 p.) _cill. en noir et en coul., couv. ill. en coul. _d26 cm
225	2		_aMethods in molecular biology _x1064-3745 _v1841
225	2		_aSpringer protocols
305			_aPublié aussi sous forme numérique avec l’e-ISBN 978-1-4939-8695-8
320			_aBibliogr. en fin de chapitres. Index
330			_aThis detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond
359	2		_b1, Filter-Aided Sample Preparation for Proteome Analysis / Wiśniewski, Jacek R. _b2, Protein Enrichment from Highly Dilute Samples with StrataClean / Bonn, Florian (et al.) _b3, Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins / Sievers, Susanne _b4, Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis / Bonn, Florian (et al.) _b5, Preparation of Bacterial Magnetosomes for Proteome Analysis / Raschdorf, Oliver (et al.) _b6, Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics / Manske, Christian (et al.) _b7, Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS / Moche, Martin (et al.) _b8, Applications of Difference Gel Electrophoresis (DIGE) in the Study of Microorganisms / Trautwein, Kathleen (et al.) _b9, Proteomic Signatures in Staphylococcus aureus / Engelmann, Susanne (et al.) _b10, How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis / Reder, Alexander (et al.) _b11, Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE / Maaß, Sandra _b12, Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses / Herbst, Florian-Alexander (et al.) _b13, Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N) / Otto, Andreas _b14, Next-Generation Trapping of Protease Substrates by Label-Free Proteomics / Lindemann, Claudia (et al.) _b15, In vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host–Pathogen Settings / Pförtner, Henrike (et al.) _b16, Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography / Soufi, Boumediene (et al.) _b17, Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation / Manteca, Angel (et al.) _b18, Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria / Rossius, Martina (et al.) _b19, Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host–Microbiota Interactions / Shah, Pranjul (et al.) _b20, Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis/Proteome Extraction from Soil Samples for Metaproteomics Studies / Chourey, Karuna (et al.) _b21, Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter / Keiblinger, Katharina M. (et al.) _b22, Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria–Invertebrate Symbioses / Hinzke, Tjorven (et al.) _b23, Correction to: Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria / Rossius, Martina (et al.)
410			_0013299697 _tMethods in molecular biology (Clifton, N.J.) _x1064-3745 _v1841
410			_0157567788 _tSpringer protocols (Print) _x1949-2448
452			_0230535461 _tMicrobial Proteomics _oMethods and Protocols _fedited by Dörte Becher _sMethods in Molecular Biology
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